By using fixed charges to engineer a strong electroosmotic flow, we achieve the unidirectional transport of natural polypeptides across nanopores. Our approach enables native proteins to be transported enzymatically and non-enzymatically in the absence of denaturant and electrophoretic tags, with potential applications for protein sequencing.
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Alfaro, J. A. et al. The emerging landscape of single-molecule protein sequencing technologies. Nat. Methods 18, 604–617 (2021). A review that describes the different approaches to protein sequencing.
Bayley, H. Nanopore sequencing: from imagination to reality. Clin. Chem. 61, 25–31 (2015). A review that describes the development of nanopore sequencing.
Yu, L. et al. Unidirectional single-file transport of full-length proteins through a nanopore. Nat. Biotechnol. 41, 1130–1139 (2023). A paper that reports the transport of proteins across a nanopore using chaotropic agents.
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This is a summary of: Sauciuc, A. et al. Translocation of linearized full-length proteins through an engineered nanopore under opposing electrophoretic force. Nat. Biotech. https://doi.org/10.1038/s41587-023-01954-x (2023).
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Electroosmotic flow across nanopores for single-molecule protein sequencing.
Nat Biotechnol (2023). https://doi.org/10.1038/s41587-023-01976-5