By using fixed charges to engineer a strong electroosmotic flow, we achieve the unidirectional transport of natural polypeptides across nanopores. Our approach enables native proteins to be transported enzymatically and non-enzymatically in the absence of denaturant and electrophoretic tags, with potential applications for protein sequencing.

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Fig. 1: Polypeptide transport across nanopores.


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This is a summary of: Sauciuc, A. et al. Translocation of linearized full-length proteins through an engineered nanopore under opposing electrophoretic force. Nat. Biotech. (2023).

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Electroosmotic flow across nanopores for single-molecule protein sequencing.
Nat Biotechnol (2023).

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